goat anti mouse nephrin (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
93
Structured Review
R&D Systems
goat anti mouse nephrin
Goat Anti Mouse Nephrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+mouse+nephrin/Mouse+Nephrin+Antibody/bio_rxiv__64898__2026__01__22__701159-205-24-27
Average 93 stars, based on 117 article reviews
Goat Anti Mouse Nephrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+mouse+nephrin/Mouse+Nephrin+Antibody/bio_rxiv__64898__2026__01__22__701159-205-24-27
Average 93 stars, based on 117 article reviews
goat anti mouse nephrin - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Orthogonal Force Balance Between Contractility and Shear Stress Governs Podocyte Dynamics"
Article Title: Orthogonal Force Balance Between Contractility and Shear Stress Governs Podocyte Dynamics
Journal: bioRxiv
doi: 10.64898/2026.01.22.701159
Figure Legend Snippet: a, Model-predicted integrin stability as a function of location from foot process periphery to center under varying shear stress conditions. Low stress (blue) shows minimal peripheral preference; increasing stress (orange to red) drives progressive peripheral accumulation with central depletion. b, Schematic of predicted integrin redistribution under mechanical stress. Integrins accumulate at foot process peripheries (green) as stress increases, with potential shape changes and edge detachment under excessive loading. c, Airyscan super-resolution imaging validates predicted pattern in healthy mouse glomerulus. Integrin α3 (red) accumulates in gaps between synaptopodin-marked foot processes (green), with nephrin marking slit diaphragms (blue). Scale bar: 1 μm. d, Relative fluorescence intensity (RFI) plot along indicated line in panel c shows integrin α3 peaks (red) localized between synaptopodin peaks (green), confirming peripheral accumulation pattern. e Expansion microscopy (4× expansion) enables single foot process resolution. Podocalyxin (membrane marker, magenta) encapsulates central synaptopodin (green), with integrin α3 (red) co-localizing at periphery. Scale bar: 1 μm. f, Quantitative analysis of straightened foot processes. Integrated RFI plot from all pixels surrounding foot processes shows central synaptopodin peak flanked by two peaks in both podocalyxin and integrin α3 channels, definitively confirming peripheral integrin localization matching model predictions.
Techniques Used: Shear, Imaging, Fluorescence, Microscopy, Membrane, Marker
Related Articles
Staining:Article Title: Engineered Sialylation of Pathogenic Antibodies In Vivo Attenuates Autoimmune Disease Article Snippet: .. For immunofluorescent tissue staining, 4μm OCT (Tissue –Tek) frozen kidneys sections were fixed in acetone and stained, where indicated, with DAPI (Biolegend) or rabbit anti-mouse IgG-Fc specific-DyLight405 (Jackson ImmunoResearch) in combination with rat anti-mouse CD41-APC (Biolegend), rat anti-mouse CD62P-PE (Biolegend), and Immunofluorescence:Article Title: Establishment of Nephrin Reporter Mice and Use for Chemical Screening Article Snippet: Frozen tissues were sliced with cryostat (CM1950, Leica Biosystems, Wetzlar, Germany) at 4 μm thickness. .. For immunofluorescence [ ], kidney sections or cultured tissues were incubated with PBS containing 10% normal donkey serum (Dako, Agilent Technologies, Santa Clara, CA, USA) and 0.3% Triton X-100 for 30 min, washed and incubated with primary antibodies (1:200 dilution) at 4°C overnight: Article Title: Establishment of Nephrin Reporter Mice and Use for Chemical Screening. Article Snippet: Frozen tissues were sliced with cryostat (CM1950, Leica Biosystems, Wetzlar, Germany) at 4 μm thickness. .. For immunofluorescence [17], kidney sections or cultured tissues were incubated with PBS containing 10% normal donkey serum (Dako, Agilent Technologies, Santa Clara, CA, USA) and 0.3% Triton X-100 for 30 min, washed and doi:10.1371/journal.pone.0157497.g001 PLOS ONE | DOI:10.1371/journal.pone.0157497 June 30, 2016 3 / 15 incubated with primary antibodies (1:200 dilution) at 4°C overnight: Cell Culture:Article Title: Establishment of Nephrin Reporter Mice and Use for Chemical Screening Article Snippet: Frozen tissues were sliced with cryostat (CM1950, Leica Biosystems, Wetzlar, Germany) at 4 μm thickness. .. For immunofluorescence [ ], kidney sections or cultured tissues were incubated with PBS containing 10% normal donkey serum (Dako, Agilent Technologies, Santa Clara, CA, USA) and 0.3% Triton X-100 for 30 min, washed and incubated with primary antibodies (1:200 dilution) at 4°C overnight: Article Title: Establishment of Nephrin Reporter Mice and Use for Chemical Screening. Article Snippet: Frozen tissues were sliced with cryostat (CM1950, Leica Biosystems, Wetzlar, Germany) at 4 μm thickness. .. For immunofluorescence [17], kidney sections or cultured tissues were incubated with PBS containing 10% normal donkey serum (Dako, Agilent Technologies, Santa Clara, CA, USA) and 0.3% Triton X-100 for 30 min, washed and doi:10.1371/journal.pone.0157497.g001 PLOS ONE | DOI:10.1371/journal.pone.0157497 June 30, 2016 3 / 15 incubated with primary antibodies (1:200 dilution) at 4°C overnight: Incubation:Article Title: Establishment of Nephrin Reporter Mice and Use for Chemical Screening Article Snippet: Frozen tissues were sliced with cryostat (CM1950, Leica Biosystems, Wetzlar, Germany) at 4 μm thickness. .. For immunofluorescence [ ], kidney sections or cultured tissues were incubated with PBS containing 10% normal donkey serum (Dako, Agilent Technologies, Santa Clara, CA, USA) and 0.3% Triton X-100 for 30 min, washed and incubated with primary antibodies (1:200 dilution) at 4°C overnight: Article Title: Establishment of Nephrin Reporter Mice and Use for Chemical Screening. Article Snippet: Frozen tissues were sliced with cryostat (CM1950, Leica Biosystems, Wetzlar, Germany) at 4 μm thickness. .. For immunofluorescence [17], kidney sections or cultured tissues were incubated with PBS containing 10% normal donkey serum (Dako, Agilent Technologies, Santa Clara, CA, USA) and 0.3% Triton X-100 for 30 min, washed and doi:10.1371/journal.pone.0157497.g001 PLOS ONE | DOI:10.1371/journal.pone.0157497 June 30, 2016 3 / 15 incubated with primary antibodies (1:200 dilution) at 4°C overnight: other:Article Title: Glycoengineering Article Snippet: Ankle joints were dissected and incubated in the fixative and decalcifier solution Cal-Ex II for 48 hrs-72 hrs (Fisher Chemical), and embedded in paraffin. |
